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Inserts encoding the neoAgs were obtained from Genscript and cloned into MSCV-IRES-Thy1.1 DEST and pBK-T-SFV-b12A utilizing BglII&SalI and Apa I sites, respectively. MSCV-IRES-Thy1.1 DEST was a gift from Anjana Rao (Addgene plasmid # 17442). Plasmids pMSCV-TMGP69-IRES-Thy1.1, pMSCV-TMGP73-IRES-Thy1.1 encoding the neoAgs and management plasmid (MSCV-IRES-Thy1.1 DEST) have been used to arrange retrovirus for transduction experiments. As a library, NLM provides access to scientific literature. Inclusion in an NLM database doesn’t indicate endorsement of, or agreement with, the contents by NLM or the National Institutes of Health. Federal government websites usually end in .gov or .mil.

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RNA sequencing was subcontracted to Macrogen Inc . Briefly, complementary DNA libraries have been constructed from High high quality RNA (1 μg) using TruSeq stranded Total RNA Sample Prep Kit. Library was subjected to paired-end sequencing utilizing the NovaSeq platform (10 Gb 100PE 100Mreads/sample).

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